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英文名称:FUNNEL PE POWDER HVY LARGE
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed
A quick RNA mini-prep for Neurospora mycelial cultures
have been developed for the processing of large quantities of material. These typically involve multiple phenol extractions (Reinert et al. 1981 Mol. Cell Biol. 1:829-836) or guanadinium isothio-cyanate/cesium chloride gradients (Chirgwin et al. 1979 Biochem 18:5294
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