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文献和实验Agarose Gel Electrophoresis for the Separation of DNA Fragments
the lid. Slowly and carefully load the DNA sample(s) into the gel (Fig. 3). An appropriate DNA size marker should always be loaded along with experimental samples. 6) Replace the lid to the gel box. The cathode (black leads) should be closer
Northern Blotting方法(Howell Lab)
Fixing Bake membrane in 80℃ vacuum oven for 20 min. UV crosslink (on optimal setting of Fisher Crosslinker) the membrane (face side up). Store between 2 pieces of chromatography paper in a plastic bag. Labelling the probe
of gel or urea is left. Stand the gel in the lower buffer reservoir so that the notched glass plate faces the top reservoir (make sure the seal is in it’s groove) and the metal plate in the back. Clamp the gel with the metal plate
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