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货号:T_70310666951
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英文名称:BOSSHEADS 27 MM D=27mm, Winkel 90° und 180°
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
polymerase (REDTaq; Sigma D4309) (for polymerase chain reaction [PCR]; see Step 45 and Discussion) dNTP set (100 mM; Invitrogen) (for PCR; see Step 45 and Discussion) Ethanol (70% and 100%) Fetal bovine serum (10% in PBS) Formaldehyde
). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
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