赛默飞世尔Thermo Fisher Comb for Ve

SDS-PAGE Gels

Preparation of PAGE gels.1. Clean glass plates with ethanol and assemble casting stand, see Biorad instruction manual2. Mix solutions for lower gel in order shown ie. TEMED last and pour into plates leaving about 2cm

SDS-PAGE

for routine analytical gels, and 1.5 mm spacers to run more sample. I usually use a comb that makes 20 wells.2. Solutions:I. 1.5 M Tris-HCl, pH 8.8II. 1 M Tris-HCl, pH 6.8III. 30% acrylamide/bisacrylamide (37.5:1). This solution can be bought commercially

Denaturing Discontinuous Polyacrylamide Gel Electrophoresis (SDS-PAGE)

until the top is reached.   Carefully place an appropriate comb into each gel, taking care not to trap any bubbles. Allow gel to polymerize for about 1 hour.   Remove the front faceplate of the caster. Carefully remove the gels