赛默飞世尔Thermo Fisher 250ml玻璃瓶,10

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

of 70‐ to 250‐ml volume (e.g., CBS Scientific GM‐150 or GM‐200 or Hoefer SG500) Magnetic stirrers and stir bars Graduated cylinder Adhesive tape 1.5‐mm Teflon combs (10‐, 15‐, or 20‐tooth

DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE

EGTA stock, pH 8.0) in 100 ml PBS, pH 7.2-7.4 Heat about 80ml H2 O to 600 C and add paraformaldehyde; add 1-2 drops of 10M NaOH to get paraformaldehyde in solution. Cool to room temperature, add 10 ml 10X PBS, adjust pH with HCl, add MgCl

Myosin Light Chain Preparation

in the cold room and stir for 1 hour at 4℃. 11.Centrifuge at 8000 rpm for 20 minutes at 4℃ using the Beckman J21-C and 500 ml prechilled polycarb bottles with inner seals *usually 14 bottles- 3 spins are necessary;rotor JA10). 12.Filter the supernatant