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文献和实验Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
samples is necessary to determine the fluorescence overlap of APC and Alexa Fluor 488 into other detectors on the flow cytometer and to set accurate fluorescence compensation. 2. Label the cell-surface proteins of interest:
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
on the same day, keep the plates away from light until it can be done. For counting spots, sensitivity and compensation are set using wells coated with either anti-IL-2 or anti-IFN- coating buffer (Step 3), in which cells were stimulated
#______________ 1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________ X μl Nuclease free H2O to 10 μl 37℃ at least 1 hr After incubation, bring reaction volume up to 50 μl before cleaning up with spin column
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