赛默飞世尔Thermo Fisher POT HYDROXI

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

of aromatic amines. Synlett. 2002:802‐804.    Miles, R.W., Samano, V., and Robins, M.J. 1995. Nucleic acid related compounds. 86. Nucleophilic functionalization of adenine, adenosine

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

thymidine 5′‐triphosphate (TTP, 8t O ). The crude sample can be analyzed on a Welchrom C18 (or Ultisil C18) reversed‐phase column (4.6 × 250 mm) measured at 260 nm at a flow of 1.0 mL/min using a linear gradient of 0% to 40% buffer B in 20 min. Buffer A: 20

Protocol for Trichloroacetic Acid (TCA) Precipitation

and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish