赛默飞世尔Thermo Fisher 18-400 CAP,

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

covalently bound through a 3′‐O ‐hemisuccinate linker (Glen Research) 4‐mL screw‐cap glass vials (Fisher) 5 µm Supelcosil LC‐18

【转贴】immunofluorescence staining

. Store at 4C to prolong fluorescence.Reagents and Supplies:PBS & TBS-T buffers:PBS: Standard PBS for mammalian cellsTBS-T: Tris HCl 20 mM, pH 7.4 NaCl 137mM Tween-20 0.1%PEM Buffer:Stock Solution for 1 Liter:Final Concentration: 400 mM Potassium PIPES, pH

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

) Chloroform (CHCl 3 , Fisher) Acetic acid (AcOH, Acros) Silica gel (60 Å, 230 to 400 mesh, Merck