赛默飞世尔Thermo Fisher STAINING BO

【转贴】immunofluorescence staining

Immunofluorescence StainingFormaldehyde fixation for cultured/cytospun cellsAll steps are performed at room temperature unless otherwise indicated.Square and rectangular coverslips may be fixed and washed in small Coplin jars, 35 mm petri dishes

将滋养层和内细胞群分别染色，TUNEL检测牛胚胎凋亡

onto slides in a small volume of glycerol.  20)  Mount a coverslip over the slides and place in a dark box at 4°C until viewing with an epifluorescence microscope.  View slides within 1-2 days.   上一篇：FAM caspase 8 and 9 binding assay

（共享）免疫组织化学染色FAQ

it before and after the actual staining is done? Answer 1. t-butanol, dioxane and tetrahydrofuran are miscible with wax, water and resinous mounting media. Of these, only t-butanol (= tertiary butyl alcohol) is suitable for ordinary use