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999
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北京泽平
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热销品大量现货,其余请咨询
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EA
货号:T_70312029837
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英文名称:X5 BOTTLE,WASH,LDPE,WIDE NECK ROUND,500ML,CLOSURE RED (WGW237P) (PACK OF 5)
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文献和实验Procedures for In Vitro Production of Bovine Embryos
size). 09-761-34 150 ml 45 mm neck bottle top (09-740-22F) Syringe filters, 0.22 m m mesh size (09-754-13) Integrid 100 mm dish (08-757-149) KimwipesLong (06-666C)Square (06-666A)Pipets 2 ml sterile pipets (13-675-3C
to give a final concentration of 0.5M NaCl, i.e., add 25ml of 4M NaCl to 500ml of our normal TTBS]. Variant 2. Coupling DNase 1 to Affigel 10 1. Wash one bottle of Affigel 10 Gel (BioRad) into a Buchner funnel with a sintered glass filter. Wash
containing 0.5 mL heptane and 0.5 mL methanol. For large collections, transfer embryos to a 500 mL bottle containing 100 mL heptane and 100 mL methanol. Devitellinize the embryos by shaking vigorously for 45 s until most of the embryos sink
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