赛默飞世尔Thermo Fisher 玻璃搅拌棒 STIRR

AMNIOTIC FLUID CULTURES ON COVERSLIPS

ml conical centrifuge tubes. If the sample is received differently, transfer to two tubes. Centrifuge the tubes for 6-8 minutes at 150 x g (900 rpm). Leaving 0.5 ml, transfer supernatant to a 15 ml centrifuge tube (this will be frozen for back-up AFP

DNA Solutions （DNA分离）

ddH2 O 1 ml 10X Mn2+/isocitrate buffer : 50 mM MnCl2, 150 mM isocitrate (Na salt), 25% glycerol in double distilled water 50 μl 1 M MnCl2 150 μl 1 M isocitrate 250 μl glycerol 550 μl ddH2 O 1 ml 10x MTBE (Modified Tris-borate-EDTA buffer

【交流】染色体免疫沉淀技术详细步骤-young lab ！！！

50 mM  1M Hepes-KOH, pH 7.5  5.0 ml 140 mM  5M NaCl  2.8 ml 1 mM  0.5M EDTA  0.2 ml 10%  50% glycerol  20.0 ml 0.5%  10% NP-40  5.0 ml 0.25%  10% Triton X-100  2.5 ml   ddH2O  64.5 ml Lysis Buffer 2 (LB2) Final Conc.  Stock  For 100 ml 10