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文献和实验Hints and precautions for the care, feeding and breeding of Neurospora
, above 30°C. We routinely make crosses on SC slants in 18 X 150 mm tubes, using foam or cotton plugs. Push-on metal or plastic caps should be avoided with cross tubes because they result in more rapid water loss and are more prone to contamination
Hints and precautions for the care, feeding and breeding of Neurospora
, 5 to 7 hours at 34°C, 11 to 12 hours at 39°C. (39°C is above the optimum.) Figure 1 . Camera lucida drawing of germinating ascospores showing the segregation of two genes in a cross of col-4 x pyr-2 . Spores were isolated on minimal agar medium
can cause the needle to shoot out like an arrow here, so keep your face etc. out of the way. Also, there are two small black rubber O rings in the assembly; make sure these don't fall out and get lost. Remove the old needle and throw it out (you will leave
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