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北京泽平
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货号:T_70314803572
中文名称:玻璃瓶
英文名称:X20 Vial 28mL Fisherbrand clear
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文献和实验RNA Extraction from Frozen Tissue Sections
. Similarly, all chemicals used for RNA should be dedicated for that purpose and handled with gloves to avoid contamination. 1. Prepare a scintillation vial (Fisher #3-341-13) for each sample containing 1.5 ml of Trizol (Invitrogen#15596-026). Exercise proper
Extraction of RNA from Frozen Sections
that are always handled while wearing gloves. Similarly, all chemicals used for RNA should be dedicated for that purpose and handled with gloves to avoid contamination. 1. Prepare a scintillation vial (Fisher #3-341-13) for each sample
μM calyculin A, 2 mM PMSF, and 10 μg/ml aprotinin). 4) Lyse for 20 min on ice and clarify lysates by centrifugation at 16,000 x g for 10 min at 4 oC 5) Preclear lysates with 30 μl of a 1:1 slurry of protein G-sepharose (Fast-Flow; Pharmacia
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