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文献和实验Procedures for In Vitro Production of Bovine Embryos
is a lot but some of these tubes of HEPES-TALP will be used later in the day.2. Add 10 ml Sp-TALP to a 15 ml conical tube. Tighten the cap and place in the incubator.3. Add 5 ml IVF-TALP to a 15 ml conical tube. Leave cap loose and place in the incubator
will do if you are very careful) to help shove the blot into the tube and to plaster it against the wall of the tube.When using glass hybridization tubes, don''t tighten the caps too much: they tighten up in the oven. Make sure the cap of the tube has a red rubber O ring
). Incubate 37° for 1-2 hour. Phenol/CIA extract as before. Chloroform extract (I often don't use isoamyl alchohol here). Add 2 volumes (~0.8 ml) 100% EtOH at room temp. Invert and flick as before, and again pick the pellet out with a pipette tip to a tube
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