赛默飞世尔Thermo Fisher SNAP CAP 10

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

Identifying Chromosomal Targets of DNA‐Binding Proteins by Sequence Tag Analysis of Genomic Enrichment (STAGE)

TE buffer, pH 8.0 ( appendix 22 ) Round A dNTP mix: 3 mM each dNTP (A, C, G, and T) in TE buffer, pH 8.0