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- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311857583
中文名称：
英文名称：COMB 10 SAMPLE, 0.5MM THICK Verti-gel mini gels
货期：热销品现货，其他请咨询
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
volume of average-sized sample. Add 0.5 vol. 8 M LiCl, mix and stand at 4°C overnight. 10. Spin at 12000g, 4°C, for 15 min in a fixed angle rotor. Thoroughly resuspend pellet in 4 ml sterile gd (glass distilled) H2 O with pasteur pipette. 11. Extract twice
the bottom of the lanes with a permanent marker. 3) Peel off tape from the bottom of the cassette. 4) Insert gel into the Mini-Cell so that you can read "10 % BIS-TRIS" when facing the gel. If only running one gel use the square plastic dam to replace the second
.6. Load 10μl- 25μl(10μl for a 15 tooth mini comb) and then overlay samples with 1 x SDS running buffer. Empty wells should have 10-15μl sample buffer added and be overlayed as with the test samples so the gel runs straight. Flood the upper chamber
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