赛默飞世尔Thermo Fisher Evaporating

Construction and Characterization of Adenovirus Vectors

the cells from the dish into the medium. Transfer to a 4-mL cryovial. x. Add 40% sucrose in PBAd to a final concentration of 4%. Vortex briefly. Use immediately or store at -80°C until use. xi

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of aggregation plate Materials:       Dissecting microscope;       Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3);       1 ml syringe with 26 G 1/2 needle of KSOM medium;       light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);       

v468 Chapter 2 Wnt活性蛋白分离及应用

(Cibacron Blue F3G-A coupledto Sepharose), ~100 mL packed into an empty column with100–200 mL of bed volume. 3. Binding Buffer: 1% (w/v) CHAPS, 150 mM KCl, and 20 mMTris-HCl, pH 7.5, sterile filtered. 4. Elution Buffer: 1% (w/v) CHAPS, 1.5 M KCl