赛默飞世尔Thermo Fisher X125 8-CAP

Cell Cultures

of an ocular grid inserted into an inverted phase contrast microscope. Cell density (cells/cm2) is then plotted on a log scale against time in culture.Exercise 12.1 - Aseptic Cell Transfers Level I Figure 12.3 Flaming a wire loop and removing cap

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

Northern Blotting方法(Howell Lab)

Briefly spin up/down in the microfuge. Following the protocol from the Amersham Multiprime Kit (RPN.1601): Add 10 µl of Buffer (solution 1, blue cap vial) Add 5 µl of Primer (solution 2, black cap vial) Add 5 µl of [a-32P]dCTP (3000