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文献和实验【转贴】immunofluorescence staining
Scientific, Cat. #6663-F10, #1 thickness 11 X 22 mm rectangular coverslips.22 X 22 mm coverslips may also be used, but will require larger volumes of reagents:Fisher Scientific, Cat. #12-520-B, #1 1/2 thickness 22 X 22 mm square coverslips.Note
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
minute washes in PBS. 10) Add the appropriate fluorescently labeled secondary antibodies in PBS for 3 hours. 11) Rinse the samples with 3x 10 minute washes in PBS 12) Just before imaging the samples on the confocal microscope
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