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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311662380
中文名称:
英文名称:50 TEST REPLACEMENT KIT FOR FREE CHLORINE 50 TEST avg
货期:热销品现货,其他请咨询




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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
solution ( 36.5%; Sigma-Aldrich 33220) Glycine (2 M; Fisher BP381) Glycogen (Roche) H2 O, nuclease-free Immune complex wash buffers (high-salt and low-salt) Prepare the high-salt and low-salt versions of this buffer
Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
HIGH RESOLUTION GENETIC FOOTPRINTING
. of annealed oligonucleotide is 5 uM. 3) LIGATE PLASMID TO REPLACEMENT OLIGONUCLEOTIDE 5 ul 10X T4 ligase buffer ul Bsg I-digested library DNA (500 ng=approx. 0.5 pmol) - volume is variable 8.0 ul kinased, annealed replacement oligonucleotide (40-50
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