赛默飞世尔Thermo Fisher X2 Spacer 1

核酸电泳工作流程——5 大主要步骤

), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

Differential Display of Cotton Transcripts

and buffer (Promega, Madison, WI) 25 mM MgCl2 alpha-[33P ]-dATP (NEN Research Products, du Pont de Nemours & Co, Boston, MA) 40% acrylamide:bisacrylamide (29:1) stock solution (Fisher Scientific, Houston, TX) TBE buffer: 0.09 M Tris