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- Thermo Fisher
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- T_701K747300-0050
- 2025年07月14日
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- 文献和实验
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_701K747300-0050
中文名称：
英文名称：DRY TUBE/CHR COLUMN 50MM
货期：热销品现货，其他请咨询
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文献和实验Modified Eberwine (antisense) RNA Amplification Protocol
not to spill. Mix by pipeting 5-10X, then transfer slurry to Phase lock gel tubes. Spin 5 minutes maximum speed (15k x g) at room temperature. Transfer aqueous phase to RNase free 1.5 ml eppendorf tube. Add 70 uL 7.5M ammonium acetate (in DEPC water
to an empty BioRad spin column. Add 1.5 ml TE. Place column in 13 x 100 mm glass test tube. Spin in clinical centrifuge (12-place fixed angle rotor) on setting 2 for ~1 min until all liquid is out of the resin (don't over centrifuge as this may damage the resin
with the syringe plunger. Carefully remove the plunger and rinse with 3 ml of TE. Remove the syringe and incubate the crushed gel slice/TE on the tube roller at 37 degrees for ~ 4 hr or overnight. 4. Dump the crushed gel slice solution to a Fisher screening column
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