赛默飞世尔Thermo Fisher X100 CELL L
IFTER 180MM HANDLE X 20MM blade (pack of 100) (Ref:9125) 货号：T_70311577692- ￥70 - 1400
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999
- 供应商：
北京泽平
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货号：T_70311577692
中文名称：
英文名称：X100 CELL LIFTER 180MM HANDLE X 20MM blade (pack of 100) (Ref:9125)
货期：热销品现货，其他请咨询
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文献和实验Co-Expression of human DNA primase p49-p58 subunits
if you can do all of the spin downs in refridgerated centrifuges.If you can't do this,then work quickly and keep all buffers ice cold. Lysis buffer: •1% Triton X-100 •50mM Tris pH 8.0 •150mM NaCl •20mM imidazole (to decrease non-specific binding of proteins to Ni beads
In Situ HYBRIDIZATION TO TISSUE SECTIONS
. Probes are stored as 5X concentrates. Precipitate with the appropriate amount of tRNA as determined below. The amount of probe used per slide is 0.3-1.0ng/ml/kb (where kb refers to the length of the unhydrolyzed probe). Resuspend the probe in 20mM
can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix
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