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- 2025年07月11日
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- 文献和实验
- 技术资料
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999
- 供应商：
北京泽平
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货号：T_70113-640-512
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英文名称：PH PAPER STRIPS 8.0-9.5
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文献和实验Northern Blotting方法(Howell Lab)
top-side down and remove bubbles between gel and paper. Surround the gel with Parafilm striPS . Soak the MagnaGraph membrane in ddH20 for 3 min and lay it on top of the gel. Place 2 pieces of chromatography paper on top of membrane. Stack
O, and loading buffer as described in Steps 1-3 of this protocol. Spun columns of oligo(dT)-cellulose are available from several manufacturers. pH paper (pH test strips) Water bath (65°C)
In Situ HYBRIDIZATION TO TISSUE SECTIONS
. Giemsa (SIGMA GS-500): Use at a 1:20 dilution in 10mM NaHPO4, pH 6.8. 2mg/ml Glycine (SIGMA G7126) in 1X PBS 0.2N HCl 2M HEPES (SIGMA H3375, FW 238.3) Heptane Immersion Oil (Fisher 12-370A, Cargille Type A) Kodak
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