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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
RSP-1T 1 1" diameter post, 1" height Newport PS-1 1 1" diameter post
Preparation of low density, collagenase-digested splenocytes Protocol
from the dish and put into 50 ml tube. Rinse dish with another 3 ml 100U/ml collagenase and also add to tube. 3. Using forceps, tease the spleens apart into small fragments. Then pipette vigorously with a 5 ml pipette. Tilt the dish, and leaving the larger
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
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