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货号:T_70312822979
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英文名称:Tube UVP white light 4W 152mm
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
overnight or -70°C for 15 min. 13. Spin 12000g, 10 min, 4°C. Wash pellet with 70 EtOH and drain. Pellet should be a light pink or white. 14. Resuspend in 0.4 ml sterile gd H2 O in a microfuge tube. Precipitate with NaOAc and EtOH as in step 12.
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
. 10) Wash 3x in PBTw. 11) Divide gonads among several 6 x 50 mm culture tubes (Pyrex tubes are probably best as they can be baked to kill RNase), one tube per hybridzation. Hybridization 12) Place gonads in 400 ul of 50% PBTw / 50
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