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文献和实验Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
Chlamydomonas in a culture tube. Prepare a vaseline mount of the culture and observe the living organisms. You'll need to use dark field at a low power (100x) to locate them, as they are very small and very fast. Zoom in on one or more cells that aren't moving
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
Naphtol As BI phosphate (Sigma N2250) (stock solution 40 mg/ml in NN-DM formamide, anhydrous, 0.5 ml aliquots kept at -20°C) 10 mg Fast Blue BB Diazonium (Sigma F3378) salt. Shake well Filter with a 45µm filter. Keep away from direct light, use
Immunohistochemistry on fixed, paraffin-embedded sections
, 0.5 ml aliquots kept at -20°C) 10 mg Fast Blue BB Diazonium (Sigma F3378) salt or Fast Red Diazonium salt Shake well Filter with a 45µm filter. Keep away from direct light, use within 5 min.
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