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999
- 供应商：
北京泽平
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货号：T_70312829167
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英文名称：HYDROMETER,DENSITY 20 C M50 RANGE 950/1000 G/ML
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文献和实验Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
Overgo Probing of High-Density Filters
2.Overnight exposures are usually all that is needed. This can be increased as the filters age. Composition For 1000 ml 1% BAS (Fraction V, Sigma) 10g 1mM EDTA 2ml 0.5M EDTA (pH8.0) 7% SDS
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