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- 2025年07月16日
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999
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北京泽平
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货号：T_70121-170P
中文名称：
英文名称：MICROCAPS 80 LAMBDA 100/PK
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文献和实验Clone Genes From a Phage Library
vector (at Eco RI). Its complexity is roughly 80,000 X an insert size of 4 to 8 kb = 320 Mbp, which is about 20-fold coverage of this yeast genome. The aim is to evenly plate out about 10,000 phage over about 10 plates. What you need in addition
Easy Way to Clone Genes From a Phage Library
the genomic DNA with Tsp509 I at several different concentrations and size-selecting pieces from 4 to 8-9 kb from each digestion to ligate into the Lambda ZapII vector (at Eco RI). Its complexity is roughly 80,000 X an insert size of 4 to 8 kb = 320 Mbp
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
vector and can not be used for in vivo excision. Primers for gt11 are available from Sigma (forward primer: #P1680, reverse primer: #P1805). Growing up host bacteria Reagents: 1M MgSO4 (100 x
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