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999
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北京泽平
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EA
货号:T_70119-041-360
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英文名称:SWAB 70MM FOAM FLX TIP PK100
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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
used in this section: PBS-glucose solution, Betadine 1) For each litter, take the two sterile 100 mm petri dishes, place them in the hood and fill with ˜10 mL of ice-cold PBS-glucose. These should then be placed in an ice bucket filled
, harvest 24-48 hrs post transfection. LipofectAMINE Transfection of NIH3T3 Cells 1. Plate 3T3 cells at 2 x 105 per 35 mm dish & let grow overnight (cells should be 50-70% confluent for transfection). 2. For each transfection, dilute: A: 8 μl
100‐foot roll, 0.040‐in. (1.02‐mm) i.d. Tygon microbore polyethylene tubing Sterile, blunt 25‐G disposable hypodermic needles
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