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文献和实验Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
that is very common in detritus in ponds and in the solid portion of sewage. The heavily granulated cytoplasm makes a very nice model for observing cytoplasmic streaming. Find the specimen using dark field mode at 40x. Chaos are very large, so this shouldn't
Stainless steel mesh screen (200 mesh=74 mm,Type 316; Tylinter,Mentor,Ohio) RPMI-1640 medium Triton X-100 Lysis Buffer: 50 mM Tris-HCl,pH 7.5 150 mM NaCl 0.02% NaN3 10 mg/ml Aprotinin 10 mg/ml Pepstatin 10 mg/ml Leupeptin 1 mM PMSF (Made fresh
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
insert is centered over the glass window, the insert membrane touches the anode. 1. Use a 60-mm polystyrene Petri dish to mark the base of the chamber. 2. Prepare a cathode (�) consisting of a 3 x 0.35-mm platinum wire
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