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999
- 供应商:
北京泽平
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- 保修期:
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EA
货号:T_70311537392
中文名称:
英文名称:X4 Indicator paper reels Fisherbrand pH 5m x 7mm range 6.4-8.0 Pack of 4
货期:热销品现货,其他请咨询




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文献和实验2-D Polyacrylamide Gel Electrophoresis
Thiourea 2 g Chaps 0.5 g Mega 10 0.5 g OBG 250 µl Triton X-100 0.25 g Tris 0.4 g DTT 500 µl Pharmalytes or IPG buffer pH 3-10 (Amersham) 500 µl - mercaptoethanol 4.Add 10 µl tributylphosphine 2 mM,under the hood (2 mM final conc.) 5.Add
Two-hybrid analysis of genetic
media lacking appropriate amino acids (e.g.-u-h Glu for bait strains): sterile glycerol solution (65% (v/v)glycerol,0.1 M MgSO4,25 mM Tris-HCl pH 7.4) 1.0 to 1.5 ml cryotubes Yeast strains freshly streaked to minimal glucose plates Sterile wooden
Speed-Vac and dry the DNA pellet for about 5-10 min, or until dry.5. Always dissolve dried DNA in 10 mM Tris-HCl, pH 7.6-8.0, 0.1 mM EDTA (termed 10:0.1 TE buffer).6. It is advisable to aliquot the DNA purified in large scale isolations (i.e. 100 ug
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