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文献和实验Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes
RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes - From R. Francis � - Revised by Min-Ho Lee, 2003 - In contrast
In Situ HYBRIDIZATION TO TISSUE SECTIONS
.C.T. (Tissue-Tek O.C.T. Compound) Paraffin (Paraplast Plus, Fisher) 8% Paraformaldehyde (Polysciences E.M. Grade 0380): Prepare fresh in 1X PBS. Dissolve 8g paraformaldehyde in 100ml buffer at 65-80 degrees C. Add 1-2 drops 50% KOH
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