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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
or into 6 mm x 50 mm culture tubes). 5) Using a 10 mg/ml stock of Proteinase K (PrK), make solutions of 25 ug/ml and 40 ug/ml PrK in PBTw. Divide the gonads in half. Digest one half of the gonads in 3 ml of 25 ug/ml PrK / PBTw for 30 min and the other half
Clone Genes From a Phage Library
vector (at Eco RI). Its complexity is roughly 80,000 X an insert size of 4 to 8 kb = 320 Mbp, which is about 20-fold coverage of this yeast genome. The aim is to evenly plate out about 10,000 phage over about 10 plates. What you need in addition
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