赛默飞世尔Thermo Fisher X100 Shell
vial Fisherbrand glass amber 11.6mm x 31.5mm (dia. x h) 2mL (pack of 100) 货号：T_70312960861- ￥70 - 1400
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- T_70312960861
- 2025年07月14日
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70312960861
中文名称：
英文名称：X100 Shell vial Fisherbrand glass amber 11.6mm x 31.5mm (dia. x h) 2mL (pack of 100)
货期：热销品现货，其他请咨询
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
. Rinse with distilled water; double distilled water and ethanol. Put 1.5 mm thick spacers on the 20 x 40 cm glass plate, put the notched glass plate on top of the spacers. Clamp the plates together on one side, top and bottom with large binder
can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix
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