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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Southern Hybridization Protocols
for 5 min in a boiling waterbathSpin briefly in a microcentrifuge to bring the contents to the bottom of the tube. Straight away put on ice.Keeping at room temperature, add in labelling buffers 10 m l and enzyme 2 m l. Add in 2.5 m l radiolabelled (dCTP
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Dissecting microscope; Acid Tyrode's (e.g. Sigma: T1788); Sterile plastic Petri dishes (100 x 15 mm); M2 and KSOM medium in 1 ml syringes; 9'' Pasteur pipettes; Mouth or Finger controlled pipette. Method: Place
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