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北京泽平
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EA
货号:T_70123-100-125
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英文名称:SUPER SANI INDV 5X8 50/PK
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文献和实验Procedures for In Vitro Production of Bovine Embryos
with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap
In Situ HYBRIDIZATION TO TISSUE SECTIONS
.C.T. (Tissue-Tek O.C.T. Compound) Paraffin (Paraplast Plus, Fisher) 8% Paraformaldehyde (Polysciences E.M. Grade 0380): Prepare fresh in 1X PBS. Dissolve 8g paraformaldehyde in 100ml buffer at 65-80 degrees C. Add 1-2 drops 50% KOH
Single and Double FISH protocols for Drosophila
); dissolve in sterile H2O, divide into 50 ul aliquots and store at -20oC) . 4. 2mg/ml glycine in PBS. 5. RNA hybridization solution: 50% formamide, 5X SSC, 100 ug/ml heparin, 100 ug/ml sonicated salmon sperm DNA and 0.1% Tween 20). Filter through a 20
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