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- 2025年07月14日
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- 技术资料
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999
- 供应商：
北京泽平
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- 保修期：
1年
- 规格：
EA
货号：T_70119-140-543
中文名称：
英文名称：LABEL,GHSCORSVE B235 16/CG 1
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文献和实验Procedures for In Vitro Production of Bovine Embryos
Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM
in 4% paraformaldehyde to give a working concentration of 10 µg/mL. Discard whatever is not used on the day of use. 6) Grid plates � Purchase from Fisher Scientific (#08-757-149) 7) Na Citrate/Triton X Solution � Dilute Na Citrate in PBS to obtain
, label culture dishes. For 1 type of tissue received, set up 8 dishes. For 2 types of tissue received, set 6 on each; for 3 or more types of tissue, set 4 on each. Centrifuge the tissue in collagenase for 6 minutes at 150 X g (900 rpm in Sorval GLC-2B
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