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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70312307058
中文名称:
英文名称:X50 CAP,BLACK PLASTIC R3/28 WITH FITTED PVDC(STERA N) LINER(PACK OF 50)
货期:热销品现货,其他请咨询




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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
Northern Blotting方法(Howell Lab)
Fixing Bake membrane in 80℃ vacuum oven for 20 min. UV crosslink (on optimal setting of Fisher Crosslinker) the membrane (face side up). Store between 2 pieces of chromatography paper in a plastic bag. Labelling the probe
in each well is suctioned out with a mouth pipette.Each base-specific sequencing reaction terminated with the short termination mix is loaded using a mouth pipette onto a 0.15 mm X 50 cm X 20 cm, denaturing 5% polyacrylamide gel and electrophoresed for 2.25 hours at 22 mA
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