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- 库存:
999
- 供应商:
北京泽平
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货号:T_70310045510
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英文名称:X100 INSERT,POLYPROPYLENE WITH PLASTIC FLANGE 250µL (PACK OF 100)
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文献和实验the top of the gel twicewith water. 4. Prepare the stacking gel by mixing 1.3 mL of 4× stackingbuffer with 3.0 mL of water, 0.8 mL of acrylamide/bis solution,30 μL of ammonium persulfate solution, and 10 μL ofTEMED. Pour the stack and insert the comb. 5. Add
RIPA, NP40 lysis buffer, Triton X100 lysis buffer. Use ~750μl/10cm plate (500μl/6cm, 250μl/well of 6-well plate). 2. Spread lysis buffer evenly over cells, let plate sit on ice for 5-10min, then scrape lysate into pre-chilled microfuge tube.
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