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999
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北京泽平
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货号:T_803317121001
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英文名称:LabServ Junior Single Channel Pipette 0.1-2.5ul,50EA/CS
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文献和实验Single and Double FISH protocols for Drosophila
to the bottom of the tube (1-3hr or overnight at 4oC) before resuspending and mounting. 10. Transfer the embryos to a clean slide in ~ 80 ul mountant and cover with a 25 X 50 mm coverslip. Seal the edges with nail polish. Slides can be stored for weeks at 4oC
phenol. 6. Extract the DNA with phenol and twice with ether, as discussed above, and then ethanol precipitate. 7. Resuspend the dried DNA in 6 ul of 10:0.1 TE for use in single-stranded Sequenase[TM] catalyzed dye-terminator sequencing reactions
(0.1 U/ul) 15 ul[alpha]-32-P-dATP (PB 10384) and [alpha]-35-S-dATP (SJ 1304) from Amersham.Dilute the Bst polymerase (BioRad 170-3406) in Bst dilution buffer.3. Proceed with the sequencing reaction as described above in steps 2-5 for single-stranded
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