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文献和实验Mapping Networks of Protein‐Mediated Physical Interactions Between Chromatin Elements
20% (w/v) sodium dodecyl sulfate (SDS; Fisher, cat. no. BP166) 20% (v/v) Triton X‐100 (VWR Scientific)
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
DpnI mediated site-directed Mutagenesis
Place 200 µl highly competent cells (1 x 108/ug efficiency or greater) DH5 a on ice. Add 1-2 µl of digested PCR reaction. Incubate on ice 15-20 minutes. Heat shock 60-90 seconds, and return to ice for 2 minutes. Add 1 ml LB
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