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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:2070-05-HR
中文名称:
英文名称:ART 300 LR HR
货期:热销品现货,其他请咨询




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文献和实验Immunohistochemistry on fixed, paraffin-embedded sections
, use a clean water bath with distilled water, and let the sections dry upright in order to facilitate adhesion between the section and the charged glass surface. (Slides: Fisher Superfrost plus cat 12-550-15). [Optional: put one teaspoon Elmers'glue
antibody. The amount will depend on the antibody and the abundance (total and relative) of the antigen, but a good starting point is 0.5μg antibody / 500μg lysate. 8) Incubate for 1-2hr at 4OC with rocking. Avoid overnight incubationsa. 9) Add 30-40μl
M scale, specifying TRITYL-ON. Deprotect the crude products in a 65°C water bath for one hr. 4. Aliquot the crude product into 200-300 µl portions and set one aside for immediate purification. Vacuum-dry the remaining aliquots, then re-suspend
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