赛默飞世尔Thermo Fisher 玻璃培养管，16 x

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

type of interest, grown under appropriate cell culture conditions with appropriate medium Cells, bacterial, competent, high-efficiency ( 5 x 109 cfu/µg DNA) XL10-Gold ultracompetent cells (Stratagene) have produced optimal results.

Counting ES cell Chromosomes

for four hours in the presence of colcemid (3.125 µl of 10 µg/ml colcemid (Gibco # 15212-012) per ml of medium). 2ml per 35 mm and 4ml per 60 mm. 3) Remove media and place it in a 15 ml Falcon tube. Wash with trypsin, and trypsinize with 1.5 ml trypsin.

原代神经细胞培养方法 Neuron Cell Culture

Materials: . MEM for neurons OR Neurobasal +B-27 . 70% ethanol . Three 60 mm culture dishes with cold L-15 medium (keep on ice) . One 50 ml tube with ~35 ml cold