赛默飞世尔Thermo Fisher 8 oz / 250

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

    Dissecting microscope;       Acid Tyrode's (e.g. Sigma: T1788);       Sterile plastic Petri dishes (100 x 15 mm);       M2 and KSOM medium in 1 ml syringes;       9'' Pasteur pipettes;       Mouth or Finger controlled pipette. Method: Place

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

with ice in the sterile culture hood.    2)      Place a 250 mL beaker with 30-40 mL of Betadine into the hood.    3)      Add 50 mL/litter of PBS-glucose into a bottle, seal and place into a 37°C water bath.    4)      For each person

Metabolic labeling Immunoprecipitation

•Expre 35 S35 S Protein labeling mix (NEN,#NEG-072),7.9mCi/ml • Transfection reagents: CaCl2 ,x2HBS,DNA •Labeling Medium: RPMI1640(Met,Cys-free; Sigma R-7513)supplemented w/ 10% dialyzed FCS,& Gln • Chase Medium: Labeling medium (above)+ 500µg