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999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:3790
中文名称:
英文名称:Matrix 1.4 mL 2D, polyproplylene, V bottom tubes, bulk, 1000/case
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
RNA purification using TRizol--TRIZOL法提取RNA
microcentrifuge tubes.b.Centrifuge for 1 minute to pellet the cells.c.Pour off the supernatant.d.Add 1 ml of TRIzol or TRI reagent to the tubes.e.Lyse cells by repetitive pipetting.f.Centrifuge homogenate at 12000 x g for 10 minutes at 4℃.g.Transfer the homogenate
Purification of dnEBNA-1/Soft from E. coli BL21 LysS
on ice ~2min between rounds Transfer to microfuge tubes; Centrifuge4℃ 10k rpm 30min Transfer supernatant to fresh tube. Column Preparation a.Resuspend matrix as 50% slurry and load closed column b.Wash twice with 5 bed volumes of TED+0.15M NaCl c
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