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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:342089-0008
中文名称:
英文名称:250 mL Bottle Narrow-Mouth HDPE w/whit
货期:热销品现货,其他请咨询




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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Dissecting microscope; Acid Tyrode's (e.g. Sigma: T1788); Sterile plastic Petri dishes (100 x 15 mm); M2 and KSOM medium in 1 ml syringes; 9'' Pasteur pipettes; Mouth or Finger controlled pipette. Method: Place
Cool growth competent E . coli
al., 1990). Chill the following: >100 ml ice-cold TB, centrifuge (GSA rotor), 500 ml centrifuge bottle, and sterile freezing tubes (eppendorf tubes w/ good caps) -Innoculate 250 ml SOB media (2 liter flask) w/ 10-12 colonies bacteria
Metabolic labeling Immunoprecipitation
•Expre 35 S35 S Protein labeling mix (NEN,#NEG-072),7.9mCi/ml • Transfection reagents: CaCl2 ,x2HBS,DNA •Labeling Medium: RPMI1640(Met,Cys-free; Sigma R-7513)supplemented w/ 10% dialyzed FCS,& Gln • Chase Medium: Labeling medium (above)+ 500µg
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