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999
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货号：2330
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英文名称：ART 200 ERGO-G RACKED ST
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed
A quick RNA mini-prep for Neurospora mycelial cultures
into bd A and frq7 A was examined for the presence of the ccg-1 gene transcript (Loros et al. 1989 Science 243:385-388). Each lane contains 10 µg of RNA (1/200 of the preparation). While the fluorescence staining of the RNA extended from the 26S
In Situ HYBRIDIZATION TO TISSUE SECTIONS
/sample to 10ml formamide, boil 1', chill and add 2.5ml 5X loading buffer (0.25% BPB, 0.35% XC, 15% Ficoll in 5X TBE) - End - labeled DNA fragments (e.g., pBR322/Hinf I) may be used as size markers - Run 200V, ‰ 4h (BPB about 2/3 down the gel) - Soak 20
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