赛默飞世尔Thermo Fisher HLT 3443B T

Oxford Biomedical Research的氧化应激及慢性炎症生物标志物检测

醛酸结合，个体间葡萄糖醛酸化的范围很大，为28-80%。因此，尿液样品要用β-葡糖醛酸酶预处理，使研究者区分个体间葡萄糖醛酸化的差异并检测总系统异前列烷。   样品用葡糖醛酸酶处理对尿样中F2-异前列烷浓度检测的影响。6个人尿样混合后，分2部分。一部分掺入10 ng/ml合成的15-F2t-异前列烷。样品用或不用葡糖醛酸酶预处理得到的数据。 三、更多氧化应激研究用产品： 1.  总抗氧化能力检测试剂盒，货号TA02，96孔板：赛默飞世尔的一份应用

A quick RNA mini-prep for Neurospora mycelial cultures

. Mortar and pestle should be washed after every sample. 4. Working quickly before powder can thaw, pour or spoon ground mycelia into 15 ml round bottom Sarstedt tube (Sarstedt tubes #60.540, Princeton, NJ) containing 8 ml of E buffer at room

RNA purification using TRizol--TRIZOL法提取RNA

to a fresh tube. b.Add 0.5 ml of isopropyl alcohol to precipitate RNA. If this RNA will be used for RT-PCR, first add 50 μl isopropyl alcohol, mix, incubate the samples at room temperature for 5 minutes and centrifuge at 12,000 x g for 10 minutes at 4oC