赛默飞世尔Thermo Fisher 透明小玻璃瓶，预先装配

Northern Blotting方法(Howell Lab)

inverted because the RNA pellets on the bottom. (DNA can be recovered from the CsCl layer.) Cut off the tube bottoms (keep inverted) and resuspend the pellet in 100 µl 0.3 M NaOAc (pH 6) buffer. Transfer to a screw-cap 1.5 ml Eppi. Wash tube

Northern Blotting方法(Howell Lab)

inverted because the RNA pellets on the bottom. (DNA can be recovered from the CsCl layer.) Cut off the tube bottoms (keep inverted) and resuspend the pellet in 100 µl 0.3 M NaOAc (pH 6) buffer. Transfer to a screw-cap 1.5 ml Eppi. Wash tube

小RNA的Northern BLOT

. Note: 300mA is the max on our power supply. 5’end-labeling of Probe:  1 μl 10x PNK buffer (fresh from NEB)  30 pmol probe DNA oligo  1.66 μl [γ -32P] ATP, 150 mCi/ml (= 250 μCi, 42 pmol) Vial